emyhc (Developmental Studies Hybridoma Bank)
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Structured Review
Developmental Studies Hybridoma Bank
emyhc
Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pm42014394-237-28-29
Average 96 stars, based on 19 article reviews
Emyhc, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 96/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/emyhc/anti-Myosin+heavy+chain/pm42014394-237-28-29
Average 96 stars, based on 19 article reviews
emyhc - by Bioz Stars,
2026-10
96/100 stars
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Staining:Article Title: HuR-mediated regulation of mTOR mRNA stability promotes the commitment of satellite cells towards myogenesis. Article Snippet: .. Muscle sections were stained with hematoxylin and eosin (H&E) or were used for immunofluorescent experiments using antibodies specific for Perilipin A/B (Sigma #P1873, 1:250), laminin (Sigma #L9393, 1:500), Article Title: Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopenia Article Snippet: .. Fixed RAW264.7, BMSCs, MuSCs/myotubes and the sections were permeabilized with 0.3% PBST for 15 minutes, followed by blocking with 5% BSA for 1 hour for immune staining for 15 min at RT, followed by incubation with primary antibody against iNOS (1:500, GB11119, Servicebio), Arginase1 (1:500, GB115724 , Servicebio), IL-6 (1:200,ab233706, abcam), OCN (1:200, GB11233, Servicebio), OSX (1:500, GB111900 , Servicebio), Pax7 (1:100, bs-22741R, Bioss), Myod1 (1:100, ab203383, Abcam, UK), other:Article Title: Hypobaric Hypoxia Ameliorates Impaired Regeneration After Diabetic Skeletal Muscle Injury by Promoting HIF-1α Signaling. Article Snippet: After washing, DyLight 549- or DyLight 488-conjugated secondary antibodies (1:1000; Jackson Immuno Research, West Grove, PA, USA) were applied for 1 h at room temperature in the dark, and nuclei were counterstained with DAPI (Invitrogen, Eugene, OR, USA). Blocking Assay:Article Title: Porcine FRZB (sFRP3) Negatively Regulates Myogenesis via the Wnt Signaling Pathway Article Snippet: .. After blocking (2 h at RT; Beyotime, Haimen, China), the samples were incubated overnight at 4 °C with primary antibodies against the following: MyHC (M4276, 1:200; Sigma-Aldrich, St. Louis, MO, USA), Article Title: Porcine FRZB (sFRP3) Negatively Regulates Myogenesis via the Wnt Signaling Pathway. Article Snippet: .. After blocking (2 h at RT; Beyotime, Haimen, China), the samples were incubated overnight at 4 ◦C with primary antibodies against the following: MyHC (M4276, 1:200; Sigma-Aldrich, St. Louis, MO, USA), Article Title: Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopenia Article Snippet: .. Fixed RAW264.7, BMSCs, MuSCs/myotubes and the sections were permeabilized with 0.3% PBST for 15 minutes, followed by blocking with 5% BSA for 1 hour for immune staining for 15 min at RT, followed by incubation with primary antibody against iNOS (1:500, GB11119, Servicebio), Arginase1 (1:500, GB115724 , Servicebio), IL-6 (1:200,ab233706, abcam), OCN (1:200, GB11233, Servicebio), OSX (1:500, GB111900 , Servicebio), Pax7 (1:100, bs-22741R, Bioss), Myod1 (1:100, ab203383, Abcam, UK), Incubation:Article Title: Porcine FRZB (sFRP3) Negatively Regulates Myogenesis via the Wnt Signaling Pathway Article Snippet: .. After blocking (2 h at RT; Beyotime, Haimen, China), the samples were incubated overnight at 4 °C with primary antibodies against the following: MyHC (M4276, 1:200; Sigma-Aldrich, St. Louis, MO, USA), Article Title: Porcine FRZB (sFRP3) Negatively Regulates Myogenesis via the Wnt Signaling Pathway. Article Snippet: .. After blocking (2 h at RT; Beyotime, Haimen, China), the samples were incubated overnight at 4 ◦C with primary antibodies against the following: MyHC (M4276, 1:200; Sigma-Aldrich, St. Louis, MO, USA), Article Title: Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopenia Article Snippet: .. Fixed RAW264.7, BMSCs, MuSCs/myotubes and the sections were permeabilized with 0.3% PBST for 15 minutes, followed by blocking with 5% BSA for 1 hour for immune staining for 15 min at RT, followed by incubation with primary antibody against iNOS (1:500, GB11119, Servicebio), Arginase1 (1:500, GB115724 , Servicebio), IL-6 (1:200,ab233706, abcam), OCN (1:200, GB11233, Servicebio), OSX (1:500, GB111900 , Servicebio), Pax7 (1:100, bs-22741R, Bioss), Myod1 (1:100, ab203383, Abcam, UK), Labeling:Article Title: Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopenia. Article Snippet: Immunofluorescence Staining: Fixed RAW264.7, BMSCs, MuSCs/myotubes and the sections were permeabilized with 0.3% PBST for 15 minutes, followed by blocking with 5% BSA for 1 hour for immune staining for 15 min at RT, followed by incubation with primary antibody against iNOS (1:500, GB11119, Servicebio), .. AR TIC LE IN PR ES S ARTICLE IN PRESS Arginase1 (1:500, GB115724, Servicebio), IL-6 (1:200,ab233706, abcam), OCN (1:200, GB11233, Servicebio), OSX (1:500, GB111900, Servicebio), Pax7 (1:100, bs-22741R, Bioss), Myod1 (1:100, ab203383, Abcam, UK), Article Title: Dual-targeted hybrid nanovesicles coordinated bone-muscle regeneration via regulating the DUSP4/p38 MAPK pathway to reverse osteosarcopenia Article Snippet: .. Fixed RAW264.7, BMSCs, MuSCs/myotubes and the sections were permeabilized with 0.3% PBST for 15 minutes, followed by blocking with 5% BSA for 1 hour for immune staining for 15 min at RT, followed by incubation with primary antibody against iNOS (1:500, GB11119, Servicebio), Arginase1 (1:500, GB115724 , Servicebio), IL-6 (1:200,ab233706, abcam), OCN (1:200, GB11233, Servicebio), OSX (1:500, GB111900 , Servicebio), Pax7 (1:100, bs-22741R, Bioss), Myod1 (1:100, ab203383, Abcam, UK), Immunolabeling:Article Title: Nicotinamide and Pyridoxine Supplementation Enhances Muscle Stem Cell Activity and Muscle Regeneration in Humans: A Randomized Placebo-Controlled Clinical Trial of High Force Eccentric Contraction Recovery in Healthy Young Men. Article Snippet: he stimulations were performed in a Kin-Com dynamomeer (Kin-COM, model 500–11, Kinetic Communicator, Chattecx, hattanooga, TN), in which each stimulation was accompanied y mechanical flexion of the knee. .. All biopsy timepoints were immunolabeled for Pax7 (DSHB, Pax7, supernatant, 1:100), myogenin (DSHB, F5D, supernatant, 1:100), |
